JRD225WB protein

WB protein

Westen blotting (WB) is a protein detection technology developed by protein gel electrophoresis and solid phase immunoassay It is a protein sample separated by SDS-PAGE electrophoresis to a solid support (nitrocellulose NC film, PVDF film), and the protein on the solid phase carrier is used as an antigen, combined with a suitable antibody, and then labeled with enzymes. Anti-binding, the analysis technique for identifying and quantifying the protein after significant color or radiation self-developing, and the sensitivity of the protein is Ng, which is widely used in medical and biological research.

1, customers inform the experiment packet, number, detect sample type, species, indicators and background design materials, please explain in advance, 123] 2, sign the contract, send samples and antibodies, if you need to provide antibodies, please explain in advance; 3. Experiment, provide anti-counterfeiting experimental results, to pay the balance, provide original film and Experimental report;
4, using the software to measure the optical density value IOD of the target protein with the internal enmisin branch, and calculate the relative expression of the eye.

Customer Precautions
1, antibodies: prior consultation, explicit antibody species and whether there is this antibody can be used, if it is not negotiated by both parties Customers buy or provide or our company;
2, specimen collection and preservation:

tissue sample fresh tissue is placed in liquid nitrogen Or is stored at -70 degrees, no less than 100 mg (about mung bean size);

Cultured cell Take a cell count than 20,000 cells in EP tube, add 0.5ml of saline or protein protectant, After mixing, it is stored in the refrigerator (-70 ° C to avoid repeated freeze-thaw); blood cell specimen 2 0.5 ml of physiological saline or protein protection after depositing blood sample or lymphocyte separation Preservation (-70 ° C can be stored for a long time to avoid repeated freeze-thaw) serum or cell culture specimens After centrifugation removes impurities, take it into the EP tube, save 15-20 above 4 ° C The day, -70 ° C can be stored for a long time, avoiding repeated freeze-thaw)
3, sample transport: one way to send the specimen tissue sample, the cell sample is transported or added to the protein protective agent Ice bag transport; Cell samples can also be sent to the culture flask (sealing is not contaminated, the culture fluid does not leak, the cells should not be too full, about 50%, filled with culture);

Serum or supernatant directly adds the ice bag (the sealing liquid does not leak, depending on the road to nearly 2-3 days).

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