JRD224WB protein detection
WB protein detection: Western blotting is a technique of detecting proteins based on protein electrophoretic separation and antigenic antibody detection. The basic principle is to transfer the protein isolated from SDS-PAGE electrophoresis to a solid support under the action of the electric field, and then detect the expression of the protein in the sample with a specific antibody. It detects the sensitivity of the protein of Ng and can be analyzed and semi-quantitative analysis of the target protein. WB protein detection: The main experimental steps are as follows:
1, sample preparation: sample background analysis, protein extraction and quantification.
2, SDS-PAGE Electrophoresis.
3, protein transfer to a solid support (NC film or PVDF film).
4, Closed, Anti-Anti-Anti - incubation.
5, chemiluminescence graphic protein, analysis results.
6, Provide a detailed experimental report.
: The items you need to provide:
1, to be inspected samples and related background descriptions.
2, specific impedance and corresponding secondary antibody.
Experiment: 1. Genomics: gene chip, SNP detection, DNA methylation detection, bioinformatics analysis
2. Transcription group: microRNA chip, lncRNA chip, expression spectrum chip, RNA-SEQ
3 Proteomics: 2D-DiGe, SiLAC, ITRAQ, TMT, Label-Free, MRM 4. Gene detection: DNA / RNA extraction, RT- PCR, Real-TimePCR
5. Protein Detection: Western Blot, Co-IP EMSA, CHIP, Immunofluorescence, ELISA
6. Pathological testing: he dyeing, special dyeing, tissue sections, immunohardization, streaming cell sorting7. Metabolite: GC-MS, LC-MS, NMR
8. Cell and animal model: primary cell, cell model, Animal model construction
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