JRD226WB Method
WB sympthematic immunoassay
Immunoassay is the protein detection technology developed by protein gel electrophoresis and solid phase immunoassay, is a non-marking of SDS-PAGE electrophoresis Protein transfer to solid phase carrier (NC film, PVDF film), re-use anti-preparation or monoclonal analysis technique for identifying and quantifying proteins, and detecting the sensitivity of the protein is Ng, which is widely used in medical, biological research. field.
Step 1 Preparation of the sample (tissue, cell, serum, oozing liquid, etc.), select a suitable WB antibody.
Step Di-protein sample preparation, the preparation of total protein, membrane protein, pulp or nuclear protein according to experimental requirements. Step Tri-SDS-PAGE gel electrophoresis separation protein transferred to a solid support support carrier (NC film, PVDF film).
Step four target proteins immunological testing, chemiluminescence display protein.
Quantitative analysis of the five strips, measuring the optical density value IOD.
WB sympthematic immunoassay project Description Delivery standard project cycle WB group immunoassay 1) Internal paragraph test;
2) Customers provide test samples and antibodies (one-to-antibody), such as requiring us to provide; results report, picture, test materials 10 - 15
Experiment: 1. Genomics: gene chip, SNP detection, DNA methylation detection, biological information analysis
2. Transcription group: MicroRNA chip, LNCRNA chip, expression spectrum chip, RNA-SEQ
3. Proteomics: 2D-DiGe, SiLAC, Itraq, TMT, Label-Free 4. Gene detection: DNA / RNA extraction, RT-PCR, Real-TimePCR
[ 123] 5. Protein Detection: Western Blot, Co-IP EMSA, CHIP, ImmunoffLight, ELISA 6. Pathological testing: he dyeing, special dyeing, tissue sections, immunocetalization, flow cytometry
7. Metabolite: GC-MS, LC-MS, NMR
8. Cell and AnimalModel: primary cell, cell model, animal model construction9.
Currently, AY Pharma, Main Experimental Technical Services, Services, Animal Models, Cell Straits, ELISA Kits, etc. Welcome major scientific research enthusiasts to come to consult!