JRD224ELISA experiment

ELISA experiment

is an important technique in immunoassay that has the characteristics of sensitive, specific, simple, fast, stable and automated operation. It is currently widely used in clinical testing and research. This Law can not only determine antibodies, but also to determine antigen in body fluids, which is also a good method for early diagnosis. The ELISA approach is also suitable for finding differential proteins after experiments such as mRNA expression spectrum chip, protein chip or proteomics (mass spectrometry), further verification of difference proteins. Indirect ELISA Experiment: After the solid phase carrier is added, the antibody sample to be tested is added to the solid phase, and then the enzyme bid is added to the substrate. Determination.

Camera ELISA Experiment: Use * antigen or antibody package by solid phase carrier, after adding antibodies or antigenic reactions to be tested, then adding the second Antigen or antibody, the amount of antigen to be tested is calculated after determination of the substrate. The antigen used in this method is generally a macromolecular antigen such as protein, or the like, and is not suitable for semi-antigen such as polypeptide.

Competition ELISA Experiment: Use a solid-phase carrier with a specific antibody package, simultaneous addition of the enzyme antigens and mixture of antigens to be tested, and to be tested Combined with the enzyme antigens compete with solid phase antibody. After determination of the substrate color, the difference between the two sets of values u200b u200bcan be calculated to be tested. This method is often used to determine small molecule antigens.

Service content provides indirect ELISA detection, sandwich ELISA assay and competition law ELISA measurement. Sample requirements

1, customers provide specimens (serum, plasma, etc.) containing samples, and we develop technical service routes according to customer needs (qualitative, quantitative).

2, it is necessary to provide an antibody to be detected and the antigen is used. 3, the detected sample is a cell culture supernatant, human and animal serum, plasma, and should be stored immediately after the sample collection, if the maintenance should be -80 to save. 4. Requirements of the sample quantity: If an indicator makes a complex hole detection, no less than 250 ul, and a single hole detection should be no less than 120 ul. It is recommended that if the customer's sample is sufficient, it provides 500UL or more.
5, after the customer's sample is collected, immediately saved as required, avoid repeated freeze-thaw. Foreign customers need to use dry ice transportation, please confirm with communication before mailing.

The results and data provide
We will provide test results and experimental reports.


Currently, AY Pharma, Main Experimental Technical Services, Services, Animal Models, Cell Straits, ELISA Kits, etc. WelcomePlayers come to consult and purchase!