JRD241 Separation and Identification of Primary Cells
forced Separation and identification experiment Experimental purpose: Experimental principles from a mixed cell population Experimental principle: Take various tissues of the animal body from the body, through various enzymes, The chelating agent or mechanical treatment is dispersed into a single cell, placed in a suitable medium, allowing the cells to survive, grow and reproduce, and identify cells by morphological activation immunity.
Experimental material instrument: ultra-clean workbench, CO2 incubator, biological inverted microscope
Experimental content Method: 1. Go to the tissue block, wash, use surgery cut into small pieces then cleaning 2. trypsin digestion, stand for 5-10 minutes, suspension 3. Centrifugation, washing, adding suitable medium culture
4. Immunohistochemistry identification
Customer provides experimental information : 1. Experimental information (supplied):
sample information u0026 nbsp ; u0026; Provide (1) Sample material Fresh tissue block (2) reagent: medium, serum, such as special ingredients, customers need, drug and dose concentration
service cycle : 1 week (according to the experimental requirements appropriate adjustment cycle)
Submit: Submit an experiment report (experimental reagent and equipment, experimental process, cell picture, etc.)
Original Generation:
1. Genomics: gene chip, SNP detection, DNA methylation detection, biological information analysis
2. Transcription group: microRNA chip, lncRNA chip, expression spectrum chip, RNA-SEQ
3. Proteomics: 2D-DiGe, SiLAC, ITRAQ, TMT, Label-Free, MRM
4. Gene detection: DNA / RNA extraction, RT -PCR, Real-TimePCR
5. Protein Detection: Western Blot, Co-IP EMSA, CHIP, Immunofluorescence, ELISA [123 6. Pathological testing: he dyeing, special dyeing, tissue sections, immunohistochemistry, flow cytosystem sorted
[123 7. Metabolite: GC-MS, LC-MS, NMR
8. Cellular and animal model: primary cell, cell model
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