JRD0012 immunohistochemical experiment testing service
immunohistochemistry immunohistochemistry testing services color principle, tissue sections or cell specimens antigen presentation and antibody binding antigen-antibody reaction and chemical recycling A anti-antibiotic (such as streptavidin, etc.) with a secondary anti-labeled biotin, fluorescein, or the like. Bonding, after the chemical component of the cell or tissue is displayed by coloring reaction or fluorescence, it can clearly see the antigen antibody reaction product that occurs in the cells under optical microscopy or fluorescence microscope, which can be capable of taking the cell climb or tissue slice. Determine the distribution and content of certain chemical components.
Main steps in immunohistochemical experimental testing
frozen slices
1, will cut the frozen slices, fixes 5 to 30 min, PBS 5 ~ 10min 3minx3
2, droplets Add 0.3% Tritonx-100, 37 ° C for 20 to 40 min, PBS wash 5 to 10 min x3
paraffin slice
1, place the paraffin slice at 60 ° C for 1 h, then according to conventional dewaxing To water
2, 0.3% Tritonx-100, 37 ° C was incubated for 20 to 40 min, and PBS washed 5 to 10 min x3
3, add 3% H2O2, 37 ° C for 5 to 30 minutes. , PBS wash 5 ~ 10min x3
4, dripping the osmus blood cleaning, 37 ° C for 5 ~ 30 min, do not wash
5, add appropriate proportional dilution, 4 ° C incubation
6, take out the slice, place for 30 ~ 60min, PBS at room temperature, 5 to 10 min X3
7, add biotinized secondary, 37 ° C 20 ~ 40min, PBS 5 to 10 min x3
8, drop Hiveytase labeled streptoxin, 37 ° C 20 ~ 40min, PBS wash 5 ~ 10 min x3
9, formulated DAB color culture, 37 ° C coloring 10-30 min, sufficient water wash
10, Suumin compound Dyeing, water is washed back
11, alcohol dehydration, diol transparent, neutral gum sealing
customers provide
1 organization: fresh or fixed (10% neutral formaldehyde, 4% polyformaldehyde) tissue Sample;
2 fineCell: Fresh (greater than 10E6) or fixed (10% neutral formaldehyde, 4% polyformaldehyde) cell sample;
3 Slices: paraffin slices, frozen slices, cell climb, smear;
4 experimental materials: Anti-anti;
1 Dyeing slice;
2 electronic picture;
3 experiment report (material, reagent, instrument, method, data analysis, experimental results);
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