Barnes Maze Test

. Similar to the water maze and eight arm maze, the Barnes maze is established with the characteristics of rodent to avoid light and love. Animals obtained is from a bright and open platform to a dark and small box located below the platform. The box is called a target box. After training, animals learn and remember the location of the target box. This model is small to the animal's stress, neither it requires fasting as an eight arm maze, and is not as strong as the water maze. Therefore, it is common in memory research. In particular, it is suitable for the studies of stress-related memory studies and the behavioral phenotype of gene knockout mice. Barnes labyrinth experiment.

2. Place the animal in the plastic drum (diameter of 20 cm, high 27cm) in the center of the labyrinth. 3. Remove the round bucket, start the timer, the experimenter observes after the blindness. The animal limbs enter the target box, which is an escape (escape) and let the animals stay 30s in the box. Every animal observed for 4 min. During this time, if animals still find the target box, remove animals from the maze, put them in the target box and stay 30s. Use this gap to clean the maze. Animals trained twice daily and 5 to 6 days.