JRD239 primary cell culture experiment
Ingenic cell culture experiment 1, the choice of organs and tissues
Upon removal of the non-organized tissue and blood. 2, in primary cell culture experiment (1) Applying Pricells primary cell separation kit I, II, III.
(2) Different Pricells primary cell separation kits can be selected depending on the source of tissue.
3,:(1) Pricells primary cell special medium
( 2) Pricells primary cell specialty additive
4, the culture and identification of cells: [123
Pricells primary cell identification kit5, the introduction of the primary cells, preservation
(1) Biography: According to the growth state of the cell, the growth of cells are generated by 1: 2 or 1: 3.
(2) Save: DMSO + Culture + Serum
Cooling in the following sequence: room temperature u0026 rar; 4 ° C (20 minutes) u0026 rarr; Refrigerator freezer (30 minutes) u0026 rarr; ultra-low temperature refrigerator (-80 ° C ) u0026 rarr; liquid nitrogen.6, the recovery of primary cells
(1) Take out the cells and quickly thawed in the warm water of 37 ° C.(2) The cell suspension was sued and added 10 times or more.
(3) The culture flask was appropriately diluted with the culture medium, and the culture flask was placed in 37 ° C CO2 the incubator was allowed to vent.We have a professional laboratory, advanced experimental equipment and experienced research technicians. Technical teams include two researchers (post-doctoral), 4 assistant researchers (Ph.D.), 15 core researchers (Master), mainly dedicated to genomics, transcriptional, proteomics, metabolic, biochemistry, pathological testing, Application and promotion of genetic testing in scientific research. Through highly subdivided, better service platforms, our customers provide comprehensive technical solutions and services. Currently, and related toMany fields such as bed medicine, tumor biology, immunology, cell biology, molecular biology, etc.
More grateful is that the company has established a 1800 square meter animal center in 2015, which can provide more professional experimental animal breeding for our customers. And modeling services.
Experiment: 1. Genomics: gene chip, SNP detection, DNA methylation detection, bioinformatics analysis
2. Transcription group: microRNA chip, lncRNA chip, expression spectrum chip, RNA-SEQ
3 Proteomics: 2D-DiGe, SiLAC, ITRAQ, TMT, Label-Free, MRM
4. Gene detection: DNA / RNA extraction, RT- PCR, Real-TimePCR
5. Protein Detection: Western Blot, Co-IP EMSA, CHIP, Immunofluorescence, ELISA
6. Pathological testing: he dyeing, special dyeing, tissue sections, immunohardization, streaming cell sorting
7. Metabolite: GC-MS, LC-MS, NMR
8. Cell and animal model: primary cell, cell model, Animal model construction 9.