JRD210RT-PCR experiment

RT-PCR experiments are referred to as reverse transcription PCR, the experimental steps include RNA extraction, reverse transcription, PCR and agarose Gel electrophoresis, the grayscale value (IOD value) of the electrophoresis strip is analyzed by software, and the relative expression amount of the target mRNA is judged by comparing the grayscale value of the innergon gene.

1. Experimental supplies preparation;

2. Sample total RNA Extraction;

3.RNA concentration detection;

4. Anti-transcription;

5. Data analysis and conclusions.

The client provides:

Cell, tissue, blood or cDNA sample.

Provided:

Electrophoresis pictures, analysis results and experimental reports.

1. Genomics: gene chip, SNP detection, DNA methylation detection, bioinformatics analysis

2. Transcriptional group: microRNA chip, LNCRNA chip, expression spectrum chip, RNA-SEQ

3. Protein group : 2D-DiGe, SiLAC, ITRAQ, TMT, Label-Free, MRM

4. Gene detection: DNA / RNA extraction, Real-TimePCR [ 5. Protein Detection: Western Blot, Co-IP EMSA, CHIP, Immunofluorescence, ELISA

6. Pathological testing: HE dyeing, special dyeing, tissue sections, immunohardization, streaming cell sorting 7. Metabolism Metal: GC-MS, LC-MS, NMR

8. Cellular and animal model: primary cell, cell model, animal model construction 9.

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