JRD208SNP experiment

Experimental Principles:

[ Single Nucleotide Polymorphisms, SNP) refers to a variation of a single nucleotide on a genome, and has an average of 1000 per 1,000 in human genome. It is a single base transition, a bit of a single base in a particular nucleotide position of genomic DNA, caused a difference between groups and individuals between the populations. Among them, the conversion and the restriction occurrence frequency, the conversion refers to the conversion between the same base, that is, the pyrid, pyrimidine and pyrimidine; the subversion refers to an alternate between the occurrence of sputum and pyrimidine. In fact, the frequency of occurrence of the conversion is mostly, and the SNP appearing in the CG sequence is frequent, and most of which CG is converted to T, the CG is used as a thymine after methylation spontaneously deamination.

Experimental process:

1 [123
) Sample genome DNA extraction; [ ) Primer design, synthesis according to different regions; ) The sample is amplified and purified.
) sequencing; [ 123]
) Statistics and Analysis. 2 ,
Formation
1 PCR [123
) PCR The product of the product; [123 ) Agarose gel electrophoresis was detected.
3 , Taqman Probe profile (quantification
1 [123 DNA [123 】 Lying and quality inspection 2 ) primer [123
/ TaqMan Probe Design and Synthesis
) Fluorescence quantification [ 123] PCR 4 ) Sequencing ) Statistics and Analysis. 4 ,
Differential Law DNA [123
2 [ 123] 3 [123 ) Amplification and extension reaction 4
) Data Statistics and Analysis. Dear customers: The company has services, proteomics services, biology Information analysis, etc. If you want to know , you can learn more about more products via the web. Information, the good-to-US service is our never-ending pursuit, welcome new and old customers to buy their own favorite products, we will be happy to serve you!