JRD206 methylation experiment

Methylation experiment

Methylation experiment

refers to the addition of a methyl group to a CPG site under the catalysis of DNA Methyltransferase (DNMTS). Cytraceine 5 u0026 RSQUO; - Carbon molecules form 5-methylcytosine (5-methylcycytosine). Typically, the highly methylated promoters inhibit the transcription of the downstream gene or enable the exogenous DNA (such as a transposon) in a silent state.

High Performance:
Ability to analyze a plurality of CPG sites over 600 bp, [ 123] It is possible to analyze the paraffin tissue of formalin treatment, No fluorescent label; High precision and high accuracy: High precision (5% CV),
The experimental results of different laboratories can be comparable, repetitive; High cost performance: with a 384-well plate The PCR reaction, the reaction volume is small, the provincial reagent,
An amplification product can be analyzed by a plurality of CPG sites; Experimental process:

1. Design and synthesizing primers; 2. Extract DNA; 3. DNA sulfite modification;

4.PCR amplification;

5.MSP method to detect electrophoresis Product or BSP method sequencing PCR amplification products; 6. Data analysis

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