JRD0048ELISA, WB, Q-PCR experiment

ELISA sampling: Serum and body fluid: -20--80 Save, -20 Degree or dry ice transport

Tissue: -20 ~ 80 degree storage, -20 or dry ice transport

Cell supernatant: -20 to 80 degrees preservation, -20 or dry ice transport Cell precipitation: -20 ~ 80 degree preservation, -20 Or dry ice transport

Each indicator is a single hole detection, one sample needs to be serum 30 ul, 3 complex apertures need 90 ul, ice Short-term transport in the box - 20 degrees (mouse suggested to take eye split blood, if the blood sample is limited, more mouse blood samples can be taken under the premise allowed by the test scheme). Third, the sample number:

Sample label:
Groups and sample labels, to be marked on the tube wall Clear, special attention of 6, 9 distinction, you can use English. Remember to provide our sample list. ELISA, WB, Q-PCR experiment
WB, Q-PCR sampling and maintenance precautions
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1 The cells were preserved with -80 degrees for a long time, and -20 degrees were temporarily stored. For Q-PCR, try to use a sterilized non-RNA enzyme, Trizon or RNA sample preservation fluid is long-term preservation. 2, immediately-80 or liquid nitrogen preservation immediately after tissue sample sample. Subsequent sampling of Q-PCRs is stored in liquid nitrogen or -80 degree environments, or stored in a sterile-free RNase-80 degree-8 degree of Trizon or RNA sample storage liquid.
Second, sample transportation

Short time 2 h left and right ice bags or liquid nitrogen transport, dry ice (dry ice can maintain -70 degrees) Sealed mailing transport. Third, the sample number

The sample label is clear, remember to provide a list of samples from our company.
ELISA, WB, Q-PCR experiment
Pathological Experimental Sampling and Experimental Precautions:

1After the animal tissue is extracted (the size of the corn granules) is quickly stored in 4% polymethylene formaldehyde, and the tubular reference numerals, tissue blocks as soon as possible, or can be cleaned with multiple formaldehyde to clean the samples, normal temperature, normal temperaturetransportation.Samples of the electron microscope were fixed to 15 ml of centrifuge tube by 2.5% glutaraldehyde, and the sample diameter was less than 2 mm, and normal temperature was stored at normal temperature.Provide our company sample list.
2, the cell sample was made into a climbing film, and the PBS infiltration preservation was stored in the cell culture plate and sealed at normal temperature.After the cell centrifugation of the electron microscope was collected 2.5% glutaraldehyde fixed, normal temperature transport.