JRD985 shuttle, dark (bright dark box) experiment

First, shuttle, DD (light dark box) experiment introduction

Avoiding experiment is utilized Animals are highly dark, protective light (clear dark shuttle), which is established for fear and memory of disgusting stimulation (such as a sufficient shock). Therefore, the evasion experiment is technically similar to the fear of implied association conditions for the experiment (rather than animals). The stimulation used is a mild foot electric shock, and the reaction occurs is where the animal escapes has been excited by electric shock. Divided into active avoidance and passive avoidance depending on the evasion of animals. The former requires animals to take the initiative to escape from hateful stimulating boxes; the latter requires animals to curb themselves without entering a hate stimulating box.

Second, shuttle, DD (lighting box) experimental experiment method

[ 123] 1. Single electric shock stimulation training (1) Put the mice into the bright box, 10S, open the door between the bright and dark boxes. Most of the lines have a strong inquiry behavior, and they are dark. Therefore, mice will quickly enter the dark box.

(2) After the mice (limbs) entered the dark box, they immediately closed the mednet and administering an electric shock. The electric shock strength can cause small currents that can cause mice retreat and vocal. According to the mouse line, the current range is from 0.2 to 0.8 mA (100V, 50 ~ 60 Hz AC), lasting 1 to 2s.

. Clean the operating box. Carry out the next animal. 2. Retention Test Electrical Stimulation Training After 24 hours, put mice back into the box, open the door, but not to shock

[123 ] III, indicator parameters Observation time, number of shuttle times, latency, bright box activity time, percentage of mutual box activity time, mindful activity time, dark box activity time Percentage, general distance, Mingbo box activity road, dark box activity road , company is located at 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008,More than 600 square meters of laboratory area. The company currently has better biology, medical technology service platform, which provides customers with medical research sample testing, cooperative research, development and production services. We have a professional laboratory, advanced experimental equipment and experienced research technicians. Technical teams include two researchers (post-doctoral), 4 assistant researchers (Ph.D.), 15 core researchers (Master), mainly dedicated to genomics, transcriptional, proteomics, metabolic, biochemistry, pathological testing, Application and promotion of genetic testing in scientific research. Through highly subdivided, better service platforms, our customers provide comprehensive technical solutions and services. Currently, and, involving clinical medicine, tumor biology, immunology, cell biology, molecular biology, etc. More grateful is that the company has established a 1800 square meter animal center in 2015, which can provide more professional experimental animal breeding for our customers. And modeling services. Time-time, through all employees' joint efforts, the company has a complete and integrated technology platform that screens upstream high-throughput to the downstream specific gene function. Really provide professional, perfect technical solutions and services