JRD994 new object identification experiment
The experiment is to explore the new object in the new object. The principle of learning memory test methods are established. This method has the characteristics of learning memory testing in free activities, and can simulate human learning memory behavior. At the same time, the flexibility of the new object (shape, size, etc.) is also allowed to test the formation of a long-term or short-term memory mechanism of the animal and the effect of acute drugs at a specific stage memory formation.
Second, new object recognition experiment
two objects in the same environment There are three objects A, B, C, where A, B objects are, and the C objects are different from the A, B objects. 1. The rats purchased were first adapted to 5-7 days in the breeding cage. 2. Three days before training and testing, 1 min every day, avoid stimulating rats to eliminate strangeness with testers. 3. 24 hours before testing or training, place the animals in the tested room and adapt to the test environment.
4. Beginning, first, as shown in Fig. 1, two objects of A, B are placed on the left and right ends of one side wall, and the rat is placed in the field, and the rat nasal nose distance is two things. The length is *. The rat was placed in 10 min and immediately opened the video device. The experimenter immediately left the test room, record the case of contact with these two objects, including the number of nose or mouth touches the number of objects and the distance object 2-3 cm Time (front paws on objects, nose sniffings, licking objects, etc.) are inhabitable objects, putting a standing or climbing to the object. After 10 min, the rats immediately put the rats back to the original rod box, and then tested after 1 hour after the rat (this period is still in the test room). It can also be tested after 24 hours, which can be adjusted according to experiments. 6. After 1 hour, the rat is taken after 1 hour, then the B object in the venue is converted to the C object, and the rat is still carrying the rats, and the nose is the same as the two objects, as shown in Fig. 2, Observing 2-5min time, similar to video equipment, the observer leaves the test room, and observes the indicators to step 4. Mainly observed the inquiry of rat on C objects.
7. Treats all experiments to send rats to the original breeding room.
Third, parameter indicator