Six arm water maze experiment

1, six-arm labyrinth experiment background Classic Morris Water Maze Escape Platform It is fixed throughout the experiment, which makes the hippocampus work memory processing process is simplified, so the detection of mild learning memory capabilities of smart mice is not sensitive. In 2000, Arendash et al. Used the hexapped radiation labyrine, which was carried out by the water versions of the hexapper radiant drought maze, and studied the spatial learning memory capability of GM. The program mainly uses the win-stage strategy, that is, once the mouse finds an escape platform, stay on the platform, the experiment ends.

Second, six-arm water maze experiment principle

Water is a stimulus or environment that is very disgusting in rodents. Rats will be eager to find escape in the pool. Their habit is to find an exit escape at the pool, but the dark and smooth vertical pool hinders this attempt. The underwater platform (1 cm for mice) set in the experiment can provide a place where a mice is available. But on huge water, forced swimming animals can't see the platform. To quickly find the platform, avoid being able to position the clues in the environment. Thus the clues in the mouse remember and their ability to position the platform are positioned with the platform positional relationship. Rawm can reflect the spatial learning and memory capability of mice. Third,

Advantages

[123 1 Sensitivity to detect mild learning memory capabilities than Morris maze. This is mainly because of the hexapper radiation labyrinth, the platform position is constant in the day of the experiment, and the platform position is changed in the daily experiment to observe the work memory of the animals. So mice have mastered this law to solve problems in experiments. 2 can judge the reference memory and can judge the work memory. 3 Avoid the trend of trend in the Morris water maze (THIGMOTAXIC Behavior, the tendency to swim by the pool). 4 Avoid experimental animals need to be deprived of food and water. 5 Avoid the impact of odor on animal behavior. 6 Avoid the significant effects of swimming capabilities (swimming speed) in the Morris water maze. 7 Avoid electric shock. 8 Do not need complex equipment, easy to work.

Fourth, is insufficient

1 year of the old body mice sometimes difficult to complete the task;

2 The difference between individuals is huge;

3 may not be a mouse to complete the task (information is stillLack of lacking); 4 is also limited by visual disorders;

5 Experiments need to be observed simultaneously to record a large amount of complex data.