High plus maze experiment

Experimental Principles Animals' exploration characteristics of the new areas and the fear of high-suspended open arms have formed contradictory conflicts to investigate animals, which are widely used in new drug development / screening / evaluation, pharmacology, toxicology, prevent medicine, neurogenic Science - Research and Computer Assistance Teaching in Multiple Disciplines, etc.

Second,

HIGH PLUS MAZE Experimental method

[ 123] The rat was placed in the center of the instrument, head toward the arm, the observer distance from the instrument center at least 1m. The number of times the experimental period (usually 5 min) and the number of times the arms and the closing arm and the two arm retaining time (the inlet and out arm standard should be strictly defined) Calculate rats to enter the number of openings and the total number of people account for the total number of times (the sum of the number of openings and the number of closed arms) and the total time (in the sum of the sum of the turnover time), as an evaluation Anxiety indicator. Usually this two indicators are highly correlated. If a drug increases the imagination of the animal (i.e., increasing the number of times the open arm and the percentage of the open arm), the drug is not changed, and the drug is considered to have an anxiolation effect. Similarly, if the drug reduces the preference of the open arm, it is considered to be anxious for anxiety without changing the total number of arms. Third, HIGH PLUS MAZE Experimental precautions

(1) In order to increase the total number of rats in rats, the rat hiding in the closed arm, usually placed in Open Field, and then placed in the instrument. The experiment 5-7d can also reduce the animals 1-2min per day and reduce the impact of stress stimphiism on this experiment. (2) The total number of arms reflects the motion activity of the animal (Locomotor Activity), and the effect of evaluating the anxiety status should be carried out without changing the total number of arms. If a handle is similar to how the animal's preference and the total number of arms of the arms are simultaneously, then it is to determine whether it is an anxiety and sedative effect, there is an Analysis of Covariance. Considering the correlation between the total number of arms and the above two anxiety indicators, PelloW and Lister recommends using a holeboard apparatus to measure the motion activity of animals, followed by experiment. (3) This experiment has some appraisal confidence (Face Validity), animals are unwilling to explore the results of the labyrinth, may be due to the results of all the functions caused by the fear caused by the aversion of the empty area and labyrinth elevation.It is not clear which two factors are dominated by the anxiety.

(4) Male or female rat or male sand rat.Each animal only detects once, and the animal falls in the experiment.If narrow flanges are placed on the circumference of the open arm, the sensitivity of the evaluation of anxiety is reduced.