Cell scratches experiment

Cell scratch experiments are a simple and easy to detect cell movement, and the experimental cost is low, which can be used to detect adherent growth tumor cells. Invasive transfer capacity.

Cell scratch experiment is an in vitro test method for simplicity, affordable study of cell migration / tumor invasion. The principle of this method is that when the cells grow to a single layer state, people in the fused single-layer cells are manufactured in a blank area, called u0026 ldquo; scratches u0026 rdquo ;. The cells of the scratched edge will gradually enter the blank area to make u0026 ldquo; scratches u0026 rdquo; hea.

Basic steps: u0026 ldquo; scratches u0026 rDQUO; manufacturing, cell migration during cell migration, and processing of post-data data.

I. Preparation: It is possible to sterilize, the ruler and the Marker pen are sterilized for 30 min before operation.

II. Process: 1. First use Marker pen on 6-well plate After the back, with a straight comparison, evenly spread horizontally, approximately 0.5 to 1 cm, cross the hole. Each hole passes at least 5 lines. 2. About 5 x 105 cells were added in the air, and the specific quantity was different due to cells, and the mastery was over 12 hours. 3. The next day with the gun head is compared to the straight ruler, try to be on the horizontal scratches behind, the gun head is vertical and cannot be tilted. 4. Wash the cells 3 times with PBS, go to the cells, and serum-free medium. 5. Put 37 degree 5% CO2 incubator, cultured. Press 0, 6, 12, 24 hours to sample, take pictures.

Characteristics: 1, the process of simulating the migration of cells in vivo.
2, It is very suitable for studying cell migration caused by interacting between cells and extracellular matrices (ECM), cells and cells.

3, compatible with microscope systems including live cell imaging, can be used to analyze interactions between cells.

4, a simple method of studying cell migration in vitro experiments.


After the cell fusion, the head is used to make scratches in the surface of the cell layer

method defects: manual manufactured u0026 ldquo; scratches u0026 rdquo; difficult *Sex and repeatability.