MiRNA sequencing experiment service

1. MiRNA sequencing experimental service introduction microorna (miRNA) is a size of about 21 u0026 mdash; 23 The base-chain small molecule RNA of the base is formed by a single-stranded RNA precursor with a single-stranded RNA precursor having a hairpin structure, and is different from siRNA (double strand) but is closely related to siRNA. MicroRNA degraded mRNA or inhibiting mRNA translation by pairing silencers (RISC) by mild composites (RISC), and the protein expression was regulated after transcriptional level (new discovery miRNA). MiRNA is quite conservative in species, and miRNA expression found in animals, plants and fungi, etc. has strict tissue specificity and timing. MiRNA has a variety of roles in cell growth and development, including regulating development, differentiation, apoptosis, and proliferation.

Currently studied miRNA is mainly RealTime-PCR, biocontrol technology, and second generation sequencing technique. Based on the miRNA sequencing of the second generation sequencing technology, millions of miRNA sequences can be obtained at a time, and those known and unknown miRNAs and expression differences in different organizations, different developmental stages, and different diseases can be quickly identified. The role of cell processes and its biological impact provide powerful tools. Second, miRNA sequencing experimental service analysis item

Standard analysis project

1) Original data quality control (denering contamination, low quality READS), data output statistics; [ 123]

2) Compared with reference sequences;

3) Comparison information of small RNA and RRNA, TRNA, SNRNA, Snorna, piRNA; 4) Comparison of known miRNAs in small RNA and miRBase;

5) Classify the small RNA according to the priority;

6) Predict new miRNA;

[ 123]

7) Difference miRNAAnalysis; 8) Differential miRNA expression cluster analysis;

9) miRNATargeting prediction analysis

10) Target gene function enrichment analysis

Analysis project(The following analysis selection results are preferably 3 ~ 5)

Target gene protein mutual network analysis

[123

miRNA pathway collaborative network analysis

according to customer requirementsIn-depth analysis of interest miRNA; Important miRNA function enrichment analysis;

III: Sample requirements

RNA sample total quantity: u0026 ge; 3 u0026 mu; g

RNA sample concentration: u0026 ge; 200 Ng / u0026 Mu; L Four, project cycle

45 working days