Shanghai T Maze Animal Experiment
Shanghai T Maze Animal Experiment
1. Rats and mice have good spatial discrimination function, soon quickly learn and accurately operate maze. Therefore, the T-shaped maze and radiocarpine are widely used to test the spatial memory capability of animals. T-shaped maze is used to study the effects of different brain regions on spatial memory. It is sensitive to certain brain structures, especially hippocampus. In addition, many drugs or toxins can enhance or impair the spatial memory of animals in T--shaped maze. Animals used in experiments include pigs, sheep, turtles, etc. in rats and mice. Shanghai T Maze Animal Experiment
2. The accuracy of animal selection is related to the interval between the two selection and the number of selection training during each training interval. The normal animal is very high in a short time (e.g., 5S), which is very accurate. The interval (eg, more than 1 h) is selected, and its selection is close to the random operation. Forced choice after training. If only a target arm is selected, the accuracy is usually very high. However, if you give multiple choices, the more the number of options, the worse accuracy. 3. Rodents have the characteristics of one-way preference. This unidirectional preference is related to the animal species and the system. For example, C57BL / 6J mice, ICR mice and Purdue-Wistar rats prefer the left side, while spague-dawley rats and Wistar rats prefer to the right. Studies have shown that more than 2/3 male spague-dawley rats prefer to the right side, and less than 1/5 of the left side. This one-way preference can affect the evaluation of animal learning memory.
4. The gate of the main arm is an important feature of the T-shaped maze. It can be used to limit the animals within the start box between two selections, or prevent the animal from selecting the maze between two selection training. Therefore, the animals should be quickly placed back into the starting box within the main manner between the two selection training. This is important, it can be animals not to explore the opposite target arm. 5. When the animal's stressful fear of maze or experimenter exceeds its desire to explore and foraging, the animal's inquiry of the maze is reduced, even in a maze, there is no way to explore the maze. This fear is characterized by excretion and urination in the maze; when catching it, animals will also make a scream. Therefore, sufficient stress adaptation is. Otherwise, if the animal does not perform the choice of the arm in the maze, it is not known that its memory is normal or weakened.