ITRAQ experiment service
iTraq experimental service Background introduction: Itraq and TMT technical principles, the difference is that The molecular structure is different. Compared to SiLAC (labeled protein), ITRAQ and TMT were labeled with peptides. According to the mass difference between the ITRAQ / TMT tag, the protein in the multi-group sample is performed by MS to perform relative quantification of the protein in multiple sets:
SiLAC is quantified by primary MS (MSL), and ITRAQ / TMT is quantified by secondary MS (MS / MS.ms2); ITRAQ / TMT can be compatible with source protein samples, and can simultaneously analyze samples of 8-10 groups, analyze high volume . iTraq experimental services
1, the protein is extracted and quantified, respectively.
2, Trypsin is enzymatically hydrolysis, extracting the protein;
[123 ] 3, the peptide samples were labeled with different mass numbers: 4, after the marking is completed, the sample is mixed, and the SCX is performed Component separation (15-20 components);
5, LC-MS analysis is performed for each component: [123 6, protein identification and quantitative analysis, screened differentially expressed protein;
[1237, biological experiment verification (Western blot, EUSA, etc.). Shanghai AY Technology Co., Ltd. was established in
2005
Year, companyAt 7865 SW Nimbus Avenue (Space 29-G) Beaverton OR 97008, laboratory area 600
Multi-square meters.The company currently has better biology, medical technology service platform, which provides customers with medical research sample testing, cooperative research, development and production services.