Pharmacokinetics experiment

Pharmacokinetics Experiments

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1. Understand the absorption, distribution, biological conversion, excretion of drugs and effects factor. Effects of Mastering the Concept of Off Elimination and the PH of the Cell Film on Drug Absorption and Distribution. 2. Familiar with drug elimination kinetics, time-to-time curve and multi-administration change 3. Pharmacological Significance of Mastering the Basic Parameters of Pharmacokinetics. Understand the model and significance of the room.

Second, the absorption and influencing factors of the drug

1. drug Absorption Absorption refers to the process of entering the blood circulation from the drug from the drug. After oral drug absorption, passenger entered the liver through the door, and some drugs entered the liver were metabolized by the hepatic drug enzyme, and the amount of drug circulation was reduced, called the deflation (First Pass Elimination). After the liver cancellation process, the relative amount and speed of the amount of the drug circulation and the actual dose are called biological utilization. There are a variety of forms in the absorption distribution of the drug and the transmembrane transport in the excretion process, but most drugs are transported by simple diffusion of physical mechanisms, and the diffusion velocity depends on the properties of the membrane, the area and the concentration gradient on both sides of the film. The nature of the drug is related. The molecule is small, the fat is large, the polarity is polar, and the non-detachable drug is easy to pass the biofilm. The dissolution of the drug is also different due to its PKA (the number of lysoms of acid **) and the pH of the solution. Non-desiranotic (molecular) drugs can be freely passed through a biofilm, ionic (dissociation) drug is not easy to pass the biofilm. Most drugs are weakly acidic or weak bases. Weak acid ** is less dissociated in an acidic environment, more molecular states, and vulnerable to biofilm; weak base **. Due to the difference between the two sides of the membrane, the amount of the film on the membrane will have a considerable difference when the distribution is equilibrated.

Pharmacokinetics

3, drug distribution and influencing factors Distribution (Distribution) refers to the process of reaching the tissue organ from the blood circulation system. Factors affecting distribution 1 The physicochemical properties of the drug itself (including molecular size, lipolysis, pkaWait). 2 drug and plasma protein binding rate: binding drugs cannot pass through the biofilm, only free drugs can be distributed to tissue. 3 organizational organ's barrier, such as blood brain barrier, placental barrier. 4 cell membranes both sides of the body fluid pH.

4, drug metabolism

Biotransformation also known as metabolism, refers to the process of chemical structural changes under the action of a variety of drug metabolic enzymes (especially hepatic enzymes) in vivo. The cytochrome P-450 enzyme system of liver microsomes is the main enzyme system that promotes drug metabolism in the liver. The hepatic drug enzyme has a limited activity, the individual difference is large, and the inhibitory and inhibitory of drugs is susceptible to drugs. Certain drugs can increase the activity of hepatic enzymes, increase the biological transformation of the drug, referred to as the vapor enzyme inducer, and vice versa, inhibitors are known.

5. Drainage of the drug

Excertion u0026 NBSP ; excretion is the process of discharging in vitro from the body. The kidney is the main organ of drug excretion. The primitive kidney excreted drug can be rejected in the renal tubule to extend the drug action time. The degree of healing is affected by the urine PH, and the renal tubule should be reduced from the rehabilitation of the drug.

Some drugs such as yellow poisonous pigs, partially in the hepatocytes and glucocytes, with bile discharged into small intestines, and free drugs after small intestinal hydrolysis Hepato-Enteral Circulation is known. When there is ocean yellow poisoning, it can be used for uglyamines, and the ugly amine can be combined with the foreign yellow poisonous monovine, and the combination is excreted with the manure, and the liver and intestinal circulation

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SPF animal laboratory can provide you with different kinds of animals such as mice, rats, rabbits. Pharmacokine (PK) experiment, and the route of administration is also diversified veins, goggles, subcutaneous, abdominal cavity.

Metabolic products identification

Pharmaceutical / toxic sample analysis ELISA, LC-MS, MS), etc.