Ming Dark Tank Experiment Services

Active avoidance and passive avoidance

[123 The avoidance experiment is the use of an animal's good darkness (Ming Dark shuttle), which is established for the fear and memory of disgusting stimulation (such as sufficient shock). Therefore, the evasion experiment is technically similar to the fear of implied association conditions for the experiment (rather than animals). The stimulation used is a mild foot electric shock, and the reaction occurs is where the animal escapes has been excited by electric shock. Divided into active avoidance and passive avoidance depending on the evasion of animals. The former requires animals to take the initiative to escape from hateful stimulating boxes; the latter requires animals to curb themselves without entering a hate stimulating box. According to the different instrument equipment used, it can be divided into shuttle avoidance, trip to avoid, Y-shaped maze avoided. Here mainly introduces the first two evaders.

First, the bright and dark box experimental service equipment

(1) shuttle avoidance experimental equipment Includes shuttle boxes, stimulating controllers and computers. Here is an ENV-010MC shuttle in MED Associates. It includes compament of two equivalent sizes (20.3cm * 15.9cm * 21.3cm). One of the compartments is wrapped in a black cloth, forming a dark box (active avoidance without a dark box). Between the two compartments is associated by an arcade (8.9cm * 11.4 cm), the door can be closed or opened by the experimenter according to the experiment. The compartment floor is a stainless steel fence (diameter: 4.8 mm rats, 3.175 mm mice). The fence floor on the side of the dark box is associated with the stimulator, and it can be applied; the Mingbo box is not associated with the stimulator, so there is no current.

(2) Turning the tower avoidance experimental device shifts the medium door of the above shuttle box, in which the MED ASSOCIATES is connected to the MED ASSOCIATES. It can be modified into a tower avoidance experiment device. Here is the trip to avoid device for the US Trip Corporate COULBOUM instrument company. It includes a transparent organic glass jump box of 15cm * 25cm * 10cm, a sound insulation box (48cm * 62cm * 24cm) and a pulse power stimulator. The fence at the bottom of the jetbox is coupled to the stimulator, and the central fixed 3.5cm * 3.5cm * 2.5cm wooden platform is fixed. When the experiment, the jetbox is placed in the soundproof box to prevent external interference.

Second, Method [123

(1) Mouse shuttle passive avoidance experiment (mouse step passive avoidance)

[123 ]1. Single electric shock stimulation training (1) Put the mice into the bright box, 10S, open the door between the bright and dark boxes. Most of the lines have a strong inquiry behavior, and they are dark. Therefore, mice will quickly enter the dark box.

(2) After the mice (limbs) entered the dark box, they immediately closed the mednet and administering an electric shock. The electric shock strength can cause small currents that can cause mice retreat and vocal. According to the mouse line, the current range is from 0.2 to 0.8 mA (100V, 50 ~ 60 Hz AC), lasting 1 to 2s.

. Clean the operating box. Carry out the next animal. 2. After 24 hours of memory maintenance test, put the mice back into the box, the door opened, but no shock. Observing time, number of shuttle times, latency, bright box activity time, percentage of Mingbo box activities, time, percentage of the overall activities, Total distance, Mingbo box activity distance, dark box activity distance.