Niki dyeing experiment
Niki dyeing experiment service Introduction Nissl Staining Solution is a neural biologist widely used a Nissl dyeing solution for paraffin or frozen slices of neuronal cells. Nissl Body in the slurry dye. Nissl staining is named by German psychiatrist and neuropathologist Franz Nissl.
Second, Niki dyeing experiment service [
Nissl dyed liquid is stained with blue purple, often used to display the brain Or the basic neural structure of the spinal cord. Nissl is small and large, indicating that nerve cell synthesis proteins are more functional; the number of NISSL small bodies will be reduced even disappeared when nerve cells are damaged. The active ingredient of Nissl dyeing liquid is an alkaline dye tar purple (CRESYL VIOLET), also known as the phenoli, which can be combined with RNA or DNA, can be stained with ribosome on crude endoplasm, or also dyed Nuclear nucleus. After dyeing, the cell body exhibits a mottled blue purple dye. Alkaline dyes such as Thionine (sulfur); Tolridine Blue (toluene blue) can also be used. Third,
1. Sample processing
[ 123] 2. Niki staining: 3-10 minutes in dyeing, 2 times in distilled water (1 second), 95% ethanol about 5S 3. Dehydration, transparent, sealing Fourth, customers
5, the company provides
Pictures, results and analysis.
Experimental period: 3-4 weeks.