Methylation experiment

Shanghai methylation experiment

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Shanghai methylation experiment DNA methylation is an important part of an apparent genetic basis, and CPG dinucleotide is made under the action of DNA methylated transferase (DNMTS). 5 u0026 u200b u200bRSQUO; - End cytosine transitions of 5 u0026 RSQUO; - methylcytosine. This DNA modifier achieves regulation of gene expression without changing the gene sequence. The optional method has a bisulfite modified sequencing method (BSP), a restriction endonuclease (COBRA), methylated specific PCR method (MS-PCR).

Second,

Shanghai customers provide u0026 nbsp

Blood, tissue or DNA sample u0026 nbsp

Measure fragment sequence or gene information (name, seed, sequence or genebank accession "

Third, delivery

[123 ] Experimental report: Detailed experimental step, cloned statistical results, PCR electrophoresis photos, sequencing results.

4,

Methylation detection service list. u0026 Nbsp ; ; u0026 nbsp

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Cycle

Substrate modified sequencing method (BSP) Solution, sulfite treatment, PCR, clonal sequencing, data processing [123 ] 40 working days Limited endonuclease (COBRA) , sulfite treatment, PCR, enzyme cut, picture analysis [ 123] 30 working days Methylated specific PCR (MS-PCR) Solution, sulfite treatment, PCR, clonal sequencing, data processing (design methyl) One pair of chemicals and non-methylated primers) ; u0026 nbsp ; u0026 nb