Fluorescence quantitative PCR experiment

Fluorescence Quantitative PCR Experiment Services Molecular Experiment

Fluorescence Quantitative PCR Experiment Services

Real-Time PCR, which belongs to a quantitative PCR (Q-PCR), perform DNA based on the increase amount of DNA during time. Quantitative analysis. Quantitative methods include quantification and relative quantification. Gene copy number changes can be detected by mRNA, microRNA, LNCRNA, and DNA levels.

II,

Service Process

1, primer design and synthesis [123 2, DNA / RNA extraction

3, reverse transcription (for RNA)

4, the machine quantitative analysis Third, Fluorescence Quantitative PCR

The customer provides

1, whole blood, tissue or cell.

2, primers, or supplied.

3, gene information: purpose gene name, species, and sequence (or genbank accession number).

Experimental report: Detailed operation step

Original data: dissolution Curve, amplification graph, CT value

project

Gene 43]

1 mutation [ 123]

2 mutations

3 working days 3 mutations 10 working days Three mutation points need to be determined according to specific scheme

gene fixed point mutation, length u0026 lt; 1.5 -2.5 kb

15 working days 2 mutations 15 workDay is required to set gene according to specific schemeDesignated mutation, length u0026 gt; 2.5 kb

Gene point mutation, length u0026 lt; 1.5 kb

According to the specific scheme Gene points mutation, length u0026 lt; 1.5 kb gene fixed point mutation, length u0026 lt; 1.5 kb gene fixed point mutation, length u0026 lt; 1.5 kb ;