Shanghai Maze Experiment Barnes Experiment

is American scholar Carol
ABARNES1979 franked for detection of the empty empty commented model. Similar to the water maze and radiocarish maze, the Barnes maze is built with rodents to avoid light and explore characteristics. Animals obtained is from a bright and open platform to a dark and small box located below the platform. The box is called a target box. After training, animals learn and remember the location of the target box. This model is small to stimulate the animal, neither like a radiocar maze, and it is not like a water maze. Therefore, it is common in memory research. In particular, it is suitable for the studies of stress-related memory studies and the behavioral phenotype of gene knockout mice. (1)
Shanghai Maze Experiment Barnes Experiment . It is a circular platform made of special medical organic plates, rotatable, diameter 122cm. 18 or 40 equal distance rounds around the platform were used in rats and mice; the diameters of the holes were 10 cm and 5 cm, respectively. One of the holes (called the target hole) is associated with a dark box (ie, the target box). Other round holes are empty and are not associated with objects. The dark box is arranged to draw in a drawer, which is convenient to take out the animals. You can not see the target box from the platform surface. Maze raised 140cm. Animals can escape to the target box through the target hole. There are also different considerations for the setting of mouse Bastos maze. For example, some will shorten the labyrinth diameter (eg 88 cm), and the number of holes is reduced (eg, 12), and the diameter of the hole is equivalent to the above. It is believed that such settings are conducive to increasing the ratio of mice. But no matter which setting is used, the experimental operation is similar. Through training, animals obtain space positioning for the target hole. (2) Shanghai
Maze Experimental Barnes Experiment Experimental Method
1. One day before the start of the experiment, the animals were placed from the target holes to the target box to adapt to 4 minutes. 2. Place the animal in the plastic drum (diameter of 20 cm, high 27 cm) in the center of the labyrinth.
3. After removing the round bucket, start the timer, the experimenter is observed after the render. The animal limbs enter the target box, which is an escape (escape) and let the animals stay 30s in the box. Every animal observed for 4 min. During this time, if animals still find the target box, remove animals from the maze, put them in the target box and stay 30s. Use this gap to clean the maze. Animals trained twice daily and 5 to 6 days. 4.From the second training, the maze randomly rotate one to several holes before each training, but the target box is always fixed in the same aspect. The purpose of this is to prevent animals from relying on odor rather than to determine the position of the target hole with memory.
Experiment records the following parameters: Exploring a hole's incubation period, reaching the incubation period of the target box and the number of error times for each animal (once the error is defined as an animal holding head or explores a non-target hole, Includes focus on exploring the same non-target hole).
(3) Precautions
1. Animal memory is weakened, mainly manifesting the number of erroneous times prior to successful evasion than the control group, followed by the incubation period of the target box; the incubation period of the exploration hole can be extended, or there is no significant change. Memory is enhanced, that is, the number of errors is reduced, and the latency of the target box is reached.
2. Animals have a great influence on the odor of the labyrinth. Therefore, in addition to rotating the materity between two training, 70% alcohol is used to clean the maze to eliminate the orientation of the residual odor to the next animal. 3. The Barnes Maze Platform is similar to the OpenField, which affects the factors (such as pharmaceutical treatment or genetic changes).
4.
The relationship characteristics of the variable mice have made it an ideal animal for Barnes maze research, but different lines of mice have a great difference in behavior in this experiment. For example, 129s6 mice rarely explore behavior in the Barnes maze, so it is difficult to find the target hole. C57BL / 6J mice have considerable exploration behavior, suitable for Barnes maze experiments. This is especially noticed in the memory research of genetically change mice. is American scholar Carol
ABARNES1979 franked for detection of the empty empty commented model. Similar to the water maze and radiocarish maze, the Barnes maze is built with rodents to avoid light and explore characteristics. Animals obtained is from a bright and open platform to a dark and small box located below the platform. The box is called a target box. After training, animals learn and remember the location of the target box. This model is small to stimulate the animal, neither like a radiocar maze, and it is not like a water maze. Therefore, it is common in memory research. In particular, it is suitable for the studies of stress-related memory studies and the behavioral phenotype of gene knockout mice.
(1) 123] Babus maze. It is a circular platform made of special medical organic plates, rotatable, diameter 122cm. 18 or 40 equal distance rounds around the platform were used in rats and mice; the diameters of the holes were 10 cm and 5 cm, respectively. One of the holes (called the target hole) is associated with a dark box (ie, the target box). Other round holes are empty and are not associated with objects. The dark box is arranged to draw in a drawer, which is convenient to take out the animals. You can not see the target box from the platform surface. Maze raised 140cm. Animals can escape to the target box through the target hole. There are also different considerations for the setting of mouse Bastos maze. For example, some will shorten the labyrinth diameter (eg 88 cm), and the number of holes is reduced (eg, 12), and the diameter of the hole is equivalent to the above. It is believed that such settings are conducive to increasing the ratio of mice. But no matter which setting is used, the experimental operation is similar. Through training, animals obtain space positioning for the target hole. (2)
Maze experiment Barnes Experimental method
1. One day before the start of the experiment, the animals were placed from the target holes to the target box to adapt to 4 minutes.
2. Place the animal in the plastic drum (diameter of 20 cm, high 27 cm) in the center of the labyrinth. 3. After removing the round bucket, start the timer, the experimenter is observed after the render. The animal limbs enter the target box, which is an escape (escape) and let the animals stay 30s in the box. Every animal observed for 4 min. During this time, if animals still find the target box, remove animals from the maze, put them in the target box and stay 30s. Use this gap to clean the maze. Animals trained twice daily and 5 to 6 days.
4. From the second training, the maze randomly rotate one to several holes before each training, but the target box is always fixed in the same aspect. The purpose of this is to prevent animals from relying on odor rather than to determine the position of the target hole with memory.
Experiment records the following parameters: Exploring a hole's incubation period, reaching the incubation period of the target box and the number of error times for each animal (once the error is defined as an animal holding head or explores a non-target hole, Includes focus on exploring the same non-target hole).
(3) Precautions 1. Animal memory is weakened, mainly manifesting the number of erroneous times prior to successful evasion than the control group, followed by the incubation period of the target box; the incubation period of the exploration hole can be extended, or there is no significant change. The reinforcement of memory is reversed, that is, the number of errors is reduced, and the latency of the target box is reached.short.
2.Animals have a great influence on the odor of the labyrinth.Therefore, in addition to rotating the materity between two training, 70% alcohol is used to clean the maze to eliminate the orientation of the residual odor to the next animal. 3.The Barnes Maze Platform is similar to the OpenField, which affects the factors (such as pharmaceutical treatment or genetic changes).
4.
The relationship characteristics of the variable mice have made it an ideal animal for Barnes maze research, but different lines of mice have a great difference in behavior in this experiment.For example, 129s6 mice rarely explore behavior in the Barnes maze, so it is difficult to find the target hole.C57BL / 6J mice have considerable exploration behavior, suitable for Barnes maze experiments.This is especially noticed in the memory research of genetically change mice.

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