JRD0010HE experiment test service

He experimental testing service, Hematoxylin and Eeosin Staining referred to as HE experiment testing services. Suichum refinement is alkaline, mainly to make the chromatin in the cellular nucleus with ribosome in the cytoplasm; Iran is an acid dye, mainly caused the ingredients in the cytoplasmic and extracellular matrix. The nature of easy being colored by alkaline or acidic dyes is called basophysical and eosinophils; if the affinity of the two dyes is not strong, neutrality is called neutral. The general tissue changes and tissue products can be shown by this staining and are commonly used in morns. The field of application
is used for the morphological structure of normal and lesions.
Technical Step
Taking paraffin section as an example, HE staining paraffin sliced u200b u200bbasic procedure
1, material and fixed
2, dehydration transparent
3, soaked waffles [ 123] 4, Slices and Patch
5, Defaxing Dyeing

Customers provide:
1. Tissue sample materials should be fresh, and immediately put the fixed liquid immediately after the body (10% Neutral formaldehyde, 4% polymethylene formaldehyde.
2, the cell sample was centrifuged to discard the supernatant to add 3% glutaraldehyde fixation.
Samples of paraffin slices, frozen slices, and cell climbs, smear, etc., sample preservation, transportation requirements See technical service document download information

Sword blunt


[123 1. Slices after dye;

2. Each Slices provide a photo; 3. Complete experiment report, including specific experimental methods, steps, reagents, instruments, etc., will be electronically or written Submit it.