Mouse live imaging animal experiment

Mouse live imaging animal experiment

Optical imaging in vivo body mainly uses two bioluminescence and fluorescence Technology. Bioluminescence is labeled cells or DNAs with luciferase genes, while fluorescence technology uses fluorescence report genes such as green fluorescent protein, red fluorescent proteins and Fitc, CY5, CY7, etc. mark.

Small animal biologically imaging technology is a high sensitivity refrigeration CCD to form a special imaging dark box and image processing software such that cell activity and genetic behavior within the living organism can be directly monitored. By recording the same group of experiments at different point in time, tracking the movement and changes of the target (marker cells and genes). Simple operation, the result is intuitive, high sensitivity, has been widely used in life science, medical research, and drug development.

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Second, Mouse live imaging animal experiment

service flow

1, Making animal model 2, cell label or animal label

3, vivo imaging

4, data processing

, mouse live imaging animal experiment

Oncology

application field [123

Create a tumor model, and the growth, metastasis of the tumor can be measured by living imaging techniques, and the reaction to the drug is measured, and the tumor is closer to the microenvironment of clinical onset. Compared to the traditional method, not only the sensitivity is increased (tiny tumor lesions), but also suitable for quantitative analysis of growth in tumor body, avoiding the differences between groups due to slaughter mice.

By biographic imaging techniques can study genes, drug action pathways, and drug targeting organs related to drug metabolism and Distribution law in vivo.