I. Elevated labyrinth experiment High plus maze is the exploration characteristics of the animals on the new environment and The fear of high-suspended open arms form contradictory conflict behavior to investigate the anxiety of animals. The elevated maze has a pair of open arms and a pair of closed arms, and rodents will tend to move in the closing arm due to the elderly, but they will be active in the open arms. At the same time, animals simultaneously produce impulse and fear, which has caused conflicts of exploration and evading to generate anxiety psychology. The anti-anxiety drug can significantly increase the number of times and time from entering the open arm, the labyrinth is high, which is equivalent to people standing on the cliff, making the experiment object fear and uneasiness. Elevated maze is widely used in new drug development / screening / evaluation, pharmacology, toxicology, preventive medicine, neuronics, animal psychology and behavioral biology, etc., is to carry out Behavioral research, especially the classic experiment of anxiety depression.

Experimental device consists of two opposite open arms (50cm long u0026 Times; L0CM width) and two relative closed arms (50cm Long u0026 Times; L0CM Width u0026 Times; 40CM Height) and Central Area (10cm u0026 Times; L0CM) are connected, 50cm from the ground, the closed arm is composed of high 40 cm blackboard. The interior of the room (80 lux) is measured. mice were placed in a maze from central grid at the beginning of the experiment, recording activity within 5 minutes. Observation indicators include: Open arms enter the number of times (with two peg entries), open arm residence time, closing arms enter the number of times, closed arm residence time. Calculate the proportion of open-end residence time, the open arm enters the number of times, and the total number of times in the elevated labyrinth. After the experiment was completed, the mice were removed, clean the arms, and sprayed the wine eliminated odor. Data analysis is performed after ANY-MAZE behavior software.

Entering the number of openings and residence time and the anxiety of the rats are related to the anxiety of rats, the less the number of openings, the residence time The shorter, the more serious the mouse is more serious.

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Experimental method

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Conditional Training (Day 1)

[123 No stimulation 180s

Sound signal 30s

Electric shock 2S [ No stimulation 20s

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Environment (2 days) [

No stimulation 180s [123

Changed environment related information (2 days + 2h)

No stimulation 180S Sound u0026 nbsP; 180S

No stimulation 60s

After changing environmental information (such as placing a partition plate in the experimental chamber, changing the light source and background noise or using other solution cleaning laboratory) The condition of the conditions of animals. The order of stimulation is determined by the experimenter.

After the experiment, the recorded data is analyzed by software analysis system. In the above experiment, the second phase did not give the stimulation but the experimental animals showed a significant environmental correlation fear. The performance is significantly reduced, but the freezing reaction is not present. In the third stage, the same animal did not show the immovable reaction after changing the environment (activity reduction), but after giving the sound signal, it was observed that the obvious conditional fear freezing reaction

[ 123] 3, forced swimming experiment

[123 1977, Porsolt Rd applied forced swimming experiment to detect antidepressants. Later, forced swimming experiments, becomes an animal model for evaluating antidepressants. The experimental method is a behavioral despair experiment method. The basic principle is that when the rat or mouse put into a limited space to swim, the swimming moment is escaping, soon become a floating state, only exposed The nostrils remain breathing, and the limbs occasionally drain to keep the body not sinking. It is actually an animal to give up the hopes of escaping. It belongs to the behavioral despair.

classic forced swimming experiments were carried out for two days. * Heavens to force rats or mice to force swimming 15 min in deep water at 25 ° C, dried after being taken out, cage. The next day, forced swimming for 5 min under the same conditions, and records the time. Mirua H will change the time to force swimming to (10 30) mini. Zhang Zhongqi et al., Etc., directly observed the time map of 4 minutes after 3 minutes of mice forced swimming in the 2nd day of the experiment. These are all modifications made to the classic experimental method. Rats or mice are not moving in judging antidepressantsIndicators. The shorter when the time is time, the stronger the antidepressive effect.

water temperature, water depth, animal, and season are four factors affecting the experiment. Kitada et al. Pointed out that the water temperature below 20 ° C is shortened. Therefore, the water temperature is often controlled at 25 to 30 ° C during the experiment. The selection of water depth should be unable to escape with animals. Generally, the water depth is (17 first 33) cm, and the water is about 10 cm in the experiment of mice. Water is too deep, the animal is difficult to use the precipitation and the tail, and it will swim and climb. The water is shallow, animals can use tail and back claws to maintain balance and affect the results of the experiment. In addition, animals in different brains are different from the sensitivity of antidepressants. Wistar-intestinal OT. Thin rats were more tongue than other strains of rats in swimming. The experiment was relatively large, and the timing of winter animals would be extended, and in the contrary. ;