Screening experiment of stabilizing cell line

Screening experiment of stabilizing cell line Service introduction The stabilized cell line refers to the cloning of exogenous DNA to have some Among the resistance of resistance, the carrier is transfected into the host cell and is integrated into the chromosome, and the resistance marks contained in the vector are screened, and the protein which can be stably expressed, or stably express the silent cell strain. Alternative physical methods (electric rotation), chemical methods (liposome, etc.), biological methods (viruses).

Second,

Screening experiment of stabilizing cell line

The expression of exogenous genes in cells can be divided into Two categories, one is instantaneous expression, and one is stable expression (also called * expression). The former exogenous DNA / RNA is not integrated into the host chromosome, although high levels of expression can be reached, but usually only for a few days. The latter exogenous DNA is integrated into the host cell chromosome, allowing the host cell to express the genetic gene. A stable cell line is established, typically screened with the target cells based on resistance marks contained in different gene carriers. Commonly used resistance marker genes have hygromycin, neomromycin and puromycin.

Third, the experimental process 1, carrier construction (expression plasmid vector, viral vector);

2, transfection / infection;

3 123]

5, the protein was again detected (qualitative / semi quantitative); 6, monoclonal cell strain stability detection.

Four, Customer provides

Cultured cells

5. The company provides:

Basic experimental steps, pictures, data analysis results and stable expression of cell line

Experiment cycle: about 3-4 At around the month, the specific needs is determined according to the content of the cell.