Shanghai cell scratches experiment

Introduction to the experiment of

is a simple and easy way to detect cell movement, and the experimental cost is low. It is used to detect invasive metastasis capabilities of adhesive growth tumor cells.

Material: 6-well plate (other can, but feel 6 holes, size Moderately), MARKER strippeter, 20 microliters of gun head (sterilization), serum medium, PBS

can be sterilized to sterilize, straight and MARKER pen is irradiated by UV (over-closed station)

1. First use the Marker pen behind the 6-well plate, with a straight comparison, uniformly scratched line, about 0.5 ~ 1cm, cross the hole horizontally. Each hole passes at least 5 lines.

At present, the company has been put into use with animal experimental centers, pathological experiment platforms,

cell experiments platform, molecular experiment platform, with better biology, medicine Technical service platforms can provide medical research samples, cooperative research, development and production services for our customers. 2. About 5 x 105 cells were added in the air, and the specific quantity was different due to cells, and the mastery was overnight.

3. The next day with a gun head is compared to a straight level, try to be on the horizontal scratches behind, the gun head is vertical and cannot be tilted.

4. The cells were washed 3 times with PBS, and the cells were disconnected, and the serum-free medium was added.

5. Put 37 degree 5% CO2 incubator, cultured. Press 0, 6, 12, 24 hours to sample, take pictures.

Cell scratches:

Generally, scratches experiments are cultured in the serum-free or late blood state, otherwise Cell proliferation cannot be ignored.

According to the vertical scratches of the 6-well plate, a number of intersections can be formed as a fixed detection point, which solves the problem that the position is not fixed before and after. 123]