Shanghai Steady Scrap Screening Experiment

Shanghai Steady Screen Screening Experiment

The stable expression of cell lines refers to the continuous stability expression of a particular gene or interfere with specific gene expression in cells. The purpose of steady cell strain is integrated into the cell chromosome, allowing the cells to express the gene long term.

The stable transverse strain is used in the genome research direction, which is convenient for long-term observation and test genes for cell function and protein interaction.

General Transfection Experiment:

The method of transfected plasmid or viral infection will construct good The carrier containing the target gene is introduced into the cell, and the respective drugs are selected for screening mixing positive clones according to the resistance marks contained in different gene carriers. Based on positive mixing clones, positive monoclons having a single cell length were obtained, and the cell line was stably transfected.

1, after transfecting plasmid, single clone method screening stabilized cell line [123 ]

Most cell transfection is less efficient. For gene over expression, if the transfection efficiency reaches 40%, the gene overgraduates can be expressed. However, for gene interference experiments, the transfection efficiency is much higher than that of gene overcurrent, and usually plasmid transfections cannot be satisfied.

The plasmid travels in the cytoplasm, quickly degraded, unable to meet longer detection items; the plasmid transfection is extremely low, and the stable cell line constructs consumer consumption, And the rate is very low, and the monoclonal plants are often needed.

2, viral infection screening stabilized cell line

The viral infection method is more convenient and efficient than the plain transfection screening monoclonal method, which is currently Mainstream stabilized cell line screening method. It is an ideal vector for preparation of stable cell lines due to its high-efficiency integration, high efficiency transcription, high efficiency expression, high-efficiency, high infection efficiency, and integration with cell chromosomes without gene rearrangement.

Service content: 1, stable: 15 generations stabilized transfected cell stable expression. 2, rapid: General control is completed within 1 month.

P; 3, economy: affordable, cost-effective. 4, quality: Strict identification of exogenous genes.