Western blot experiment

Western Blot Technology The step is the protein sample separated by SDS-PAGE, transferred to a solid carrier (e.g., a cellulose thin film), the protein or polypeptide on the solid phase carrier as an antigen, and the corresponding antibody is immunogeneted, and the enzyme or The second antibody labeled in the isotope is reacted, and the protein component expressed by the primer is developed or emitted from the primer to detect the specimens of electrophoresis. Since Western blot detection technique combines a variety of characteristics such as high resolution and solid phase immunization assay, 1-5 NG (low can go to 10-100 pg) medium size The target protein. At the same time, Western Blot technology is also widely used in detecting protein levels.

Western blot experiment

1, semi-quantitative or qualitative detection of the expression of the target protein in the cell or tissue; 2, the target protein is detected from the protein mixture;

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3, subsequent analysis of protein-protein, protein-DNA and protein -RNA interaction, combined with component separation technology, detecting the positioning of the target protein, is immunofluorescence

4, in the post genomic era, will cooperate with other techniques such as mass spectrometry and protein chips to play a more important role.

Service flow

Western Blot is one of the important methods of detecting proteins with antibody. Western Blot's operational steps mainly include: protein sample collection, electrophoresis, membrane, enclosure, an anti-incubation, secondary anti-incubation, substrate color development.