Shanghai overexpressed slow virus construction and packaging experiment

Shanghai overexpressed slow virus construction and packaging experiment

Shanghai overexpressed slow virus construction and packaging experiment Characteristics: [ 123] 1.1 Direct packaging into fake viral particles, infectious effects on splitting and non-divide cracked cells, suitable for cells in RNAi and in vivo experiments (such as neuronal cells, stem cells or other original Genetric cells).

1.2 can obtain a variety of cell lines that stably express a particular gene in a short period of time.

1.3 can be used in gene knockout, gene therapy and transgenic animal studies.

1.4 Easy to operate without transfection reagents.

1.5 can prepare a variety of tags according to customer needs.

2,

Lentivirus is a kind of reverse transcriptional virus. The constructed siRNA / miRNA lentiviral vector, with a chemically synthesized siRNA and a normal siRNA vector constructed by the instantaneous expression vector, on the one hand, an inherction, an in-one replacement instantaneous expression vector, on the other hand, Lentivirus-siRNA cloning passes through a slow virus package After the system is packaged, it can be used to infect cell lines that rely on conventional transfection reagents, such as primary cells, suspended cells, and non-divided cells, and can be integrated into the genome of infected cells after infection. Time stability expression. 3, overexpression of lentiviral construction and packaging process:

3.1 Construction and plasmid purification extraction of a slow viral expression vector containing a target gene .

Note: The customer can also provide a constructive recombinant lentiviral expression vector.

3.2 Slow viral expression vector and packaging system co-transfected viral packaging cells. 3.3 Collect viral fluids.

3.4 Purification and concentrated viruses.

3.5 Dispensing, - 80 OC Save.

3.6 Titness assay and sterile detection.