Shanghai chromatin immunoassate precipitation experiment

Shanghai chromatin immunocandemonized experiment

The genomic DNA of eukaryotes exists in the form of chromatin, and the interaction of protein and DNA in chromatin environments is elucising The basic pathway of nuclear biological gene expression mechanism. Compared to conventional EMSA techniques, chromatin immunoprecipitation technology (CHIP) can truly fully reflect the regulatory protein binding to the DNA sequence, which is a better way to study in vivo DNA and protein interactions.

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[123 ] Shanghai chromatin immunoprecipitation experiment

The protein-DNA complex of formaldehyde is fixed in a living cell state, and the ultrasound is randomly cut into dyeing within the length range. A small fragment, then adding a destination protein antibody, by an antibody precipitated a target protein-DNA complex, an enriched target protein-DNA complex is obtained by elution method, and the DNA fragment that is specifically enriched in the purpose of the purpose protein is carried out. Purification and detection (PCR Analysis) of the disconnection (PCR analysis) of the disciplines were obtained.

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2. Transcription regulation analysis

3.Study on drug development

4. Silica genetic study

5. DNA damage and apoptosis