Shanghai Luminex Chip Analysis Technology Experiment

Luminex chip analysis technology experiment Experiment introduction

Luminex XMap (Multi-Analyte Profiling) Technology, also known as Fluorescence technology, liquid chip. The technique is based on antibody, and the fluorescence coding microspheres are core, integrated with laser detection, application fluid, high-speed digital signal, and computer algorithm, and realizes high-throughput detection of multi-factors.

Currently, Luminex XMAP technology has been widely used in clinical diagnosis, biopharmaceutical, biomedical research, etc., which has become an important development direction of medical diagnostics and disease mechanisms.

Shanghai Luminex chip analysis technology experiment Experimental Principles

Putting the diameter It is 5.6 u0026 Mu; the spatial beyrene ball (or 6.5 u0026 Mu; M magnetic beads) encodes the fluorescent dyeing method, and obtains up to 500 different characteristic fluorescence microspheres by adjusting different ratios of the fluorescent dye. Each coded microsphere is covalently crosslinked to capture molecules such as antigen, antibodies or nucleic acid probes such as a particular detector. After mixing the microspheres, the microspheres are added, and the target molecules in the suspension are specifically bonded to the capture molecules crosslinked by the microsphere surface, and the reaction pores are completed simultaneously with 500 different detection reactions. After detection with LUMINEX 200 or FLEXMAP 3D, the instrument passes the encoding of two other microspheres and detects the fluorescence intensity of the molecules on the microspheres, respectively, thereby realizing quantitative analysis of the purpose of the purpose.

Third, Shanghai Luminex Chip Analysis Technology Experiment Sample Requirements

1, Single sample volume u0026 ge; 100 ul, if the same sample needs to be detected, the sample amount needs to be increased accordingly.

2, the sample is a cell culture supernatant, a cell or tissue lysate, requiring at least 2 ml / kit blank medium or lysate as a background control.

3, the sample needs to be sent with dry ice to keep the refrigeration in the transportation process. Third, service flow

Pre-sales support (kit selection and order).

Sample processing + experiment analysis.

Biometric analysis (significant difference analysis, correlation analysis, ROC curve, Kaplan-Meier survival analysis, etc.).

1, the volume of a single sample u0026 ge; 100 UL, if the same as the sameYou need to detect multiple KIT, the sample size needs to be increased accordingly.

2, the sample is a cell culture supernatant, a cell or tissue lysate, requiring at least 2 ml / kit blank medium or lysate as a background control.

3, the sample needs to be sent with dry ice to keep the refrigeration in the transportation process.

Experimental report, including experimental processes, instrument methods, raw data and picturesWait.

Cycle: 2-3 months