JRD0011 immunohistochemical detection service
First, immunohistochemical detection services Service introduction [123 ] Tissue and cells of tissue sections or cell specimens in tissue slices or cell specimens, referred to as immunohistochemistry techniques or immunocytochemistry Immunocytochemistry technology. The immunohistochemistry is characterized by strong specificity, accurate positioning, and thus it can be clearly related to cell positioning, subcellular positioning and multi-protein between the protein. Immunohistochemistry is widely used in medical applications, especially in clinical tumor diagnosis and identification diagnosis. It is applied to a differential diagnosis of low dividing or undifferentiated tumors, and the accuracy can reach 50% -75%.
according to the antigen antibody reaction and Principles of chemical color development, antigenic and primary antibody binding in tissue sections or cell specimens, then react with primary anti-labeled biotin, fluorescein, or the like, and then display cells or tissues after coloring reaction or fluorescence The chemical composition can clearly see the antigen antibody reaction product of the cells under an optical microscope or a fluorescent microscope, thereby determining the distribution and content of certain chemical components on the cell climb or tissue slice. 3, experimental process
Production of paraffin slice; dewaxing and hydration of paraffin slice; block endogenousity Peroxidase activity;
antigen repair; Closed non-specific protein; An anti-incubation;
sp (streptavidin - Peroxidase) reaction; DAB color; reset, dehydrated, transparent, and seal.
Mirror check, take pictures.
Fourth, customers provide
tissue or cell, one anti-(available)
Five, immunohistochemical detection service company provides
Experimental steps, result picture ,Result analysis. Experiment cycle: 1-2 weeks.