Shanghai HE dyeing experiment
Shanghai HE dyeing experiment
HE staining step: generally contains the steps of fixing, dehydration transparent, soaking wax embedding, slice and patch, dewaxing, dehydrated transparent, sealed, and other steps.
He Dyeing Service Content1. Buried, slice, dyeing, photo, etc.
2. Other special staining, Prusson dyeing, masson dyeing, etc. [ 123]
Shanghai HE Dyeing Experimental Principle
The nature of easy being alkaline or acidic dyes is called Basophilia and acidophilia; and the alkaline dye and acidic dye affinity The relatively weak phenomenon is called neutrainilia.
There are many types of proteins within the tissue constituting the amino acid of the protein, and they have different equidistors. In the normal staining, the acid-alkalinity of the dyed liquid is about pH 6, and the acidic substances within the cells such as nucleus, the coarse inner texture and the transparent cartilage matrix in the adenocyte and nerve cells are stained. Base is known as basward. Other proteins in cytoplasm, such as hemoglobin, eosinophilic particles and collagen fibers and muscle fibers, such as hemoglobin, and muscle fibers, such as acidic dye, referred to as acid dye. If the pH is changed, the pH is increased, the material that is stained with acid dye can be changed to be bactericity; the pH is lowered, the substance of the alkaline dye is dyed. Eosinophilic. Therefore, the pH of the dyeing liquid can affect the reaction of the dye.
Deoxyribonucleic acid (DNA), phosphate groups on two chains, with negative charge, acidic, and it is easy to bind to ionic bonds with positively charged tumi fine dyes. Sumu is blue in the alkaline solution, so the nucleus is dyed in blue. Yihong Y is a chemically synthetic acid dye that is separated from a negative charge in water, combined with the amino positive charge of the protein, binds to the cytosols, cytoplasm, red blood cells, muscles, connective tissue, and Emoy. The et al. Is dyed to different degrees of red or pink, and the blue nuclear nucleation is stark contrast. Inghong is a good dye of cytoplasm.
Due to different ingredients of tissue or cells, the affinity of Sumu is different and the staining properties are different. After the sow, the nuclei and calcium salt mucus are blue, and the hydrochloride can be used as a weak alkaline solution, such as the treatment, which can make the nucleus and other ingredients such as dark blue and cytoplasm. The cytosol dye irritation is used to make the various components of the cytosols in a deep shade. Therefore, all kinds of organizational or cellular components and lesions can be shown
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