Shanghai scanning electron microscopy experiment
Shanghai scanning electron microscope experiment
Scanning electron microscopy is a more modern cell biology research tool in 1965, it It has the characteristics of simple structure, magnification of magnification, high resolution, and large depth of field. In recent years, scanning electron microscopy has been widely used in the field of biology, medicine, and other subjects.
1) The basic requirements of the material
and the preparation of transmission electron microscopy samples. However, for the scanning electron microscope, the sample can be slightly larger, the area is up to 8mm u0026 Times; 8mm, the thickness is 5 mm. For volatile samples such as blood vessels, gastrointestinal mucosa, etc., can be fixed to the surface of the tissue to be observed to sufficient exposure of the tissue surface.
2) Cleaning
The portion observed by scanning electron microscopy is often the surface of the sample, ie the tissue free surface. Since the sample is taken from the living tissue, there is often blood, tissue liquid or mucus adhesion thereof, which will cover the surface structure of the sample, affect observation. Therefore, before the sample is fixed, the attachment is cleaned clean. The cleaning method has the following:
. Clean with an equal infiltrated physiological saline or buffer;
b. Wash with 5% soda;
c. Treatment with ultrasonic oscillating or enzyme digestion. For example, the mucus of the intestinal mucosa is cleaned, and the following method can be used:
Cleaning liquid formulation: hyaluronic acid enzyme 300 ug u0026 alpha; - chymotryrin 10 mg Physiological saline 100 ml of the cleaning liquid pH of 5.5 to 6. The cleaning method is to soak the sample in a wellned cleaning liquid, and the soaking bubble is oscillated for 30 minutes, and then washed 3 times with double evaporation. No matter which cleaning method, do not damage the sample when cleaning.
3) The reagents used in
fixed to fixes the same, commonly used glutaraldehyde and ferric acid double fixation. Since the sample is large, the fixed time should be appropriately extended. It is also possible to fix it with fast freezing.
4) Dehydration
Samples are rinsed after the concentration of concentrations of alcohol or acetone dehydrated with step by step, and then enters the intermediate fluid, and is generally used as an intermediate liquid with acetate. Scanning electron microscopy biological sample preparation technology Most biological samples contain moisture, and it is more soft, so before the scanning electron microscope is observed,Products have corresponding processing.The main requirements for the preparation of the scanning electron microscopy are: as much as possible to store the surface structure of the sample, no deformation and pollution, the sample is dry and there is good conductivity.
Shanghai scanning electron microscope experiment
Company providesBasic experimental steps, drugs, sample processing, pictures and picture analysis
Experiment cycle: 3-4 weeks