Sumu-Inghong Dyeing Experiment
木 精 实 [
Sumu-Eros, referred to as HE dyeing method, is a wide range of cells and histological students of biology and medicine. Dyeing method. A routine dyeing method is referred to in pathological laboratories. Diagnosis, teaching and research of pathological cells and histology are allocated to observe the morphological structure of normal and lesion tissue by HE staining. (1) Basic Principle of HE Dyeing ( Sumu u0026 Mdash; Iraqi Dyeing Experiment ) )
[ 1. The principle of nuclear staining
chromatin in the nucleus is mainly deoxyribonucleic acid (DNA), DNA double helix structure, phosphoric acid on both strands Based on the base, negative charge, acidic, and it is easy to bind to ionic or hydrogen bonds with positively charged huki-bonded dyes. Sumu is blue in the alkaline dye, so the nuclear nuclear is dyed in blue.
2. The principle of cytoplasm dye
The main ingredient in the cytoplasm is protein, adding acetic acid in the dyeing liquid to bring the cytoplasm with positive charge (cation), Dye staining with negative charge (anion). Ingry Y is a chemically synthetic acid dye that is separated into negative charge, combined with amino positive charge (cation) of the protein, which combines the amino positive charge (cation) of the protein to make cytosols, cytoplasm, erythrics, muscles, connective tissue, Emie Red particles or the like have been dyed different degrees of red or pink, form a distinct contrast with blue nucleus. Inghong is a good dye of cytoplasm.
The customer provides
Organization or paraffin specimen.
Suichum u0026 Mdash; Inghong dyeing experiment The company provides
Basic experimental steps, pictures, data analysis results.
Experimental cycle: 1 week, specifically, according to experimental content.