Transmission electron microscopy test experiment

Transmission electron microscopy detection lap by the sample, by the objective mirror, like the mirror, and then enlarged by the intermediate mirror and the projection mirror, imaging the fine material structure of the fluorescent screen or photographic ring; therefore The inner layer material is also seen while seeing the image of the surface. The specimen is made into ultra-thin slice (50 ~ 100 nm).

First, transmitting electron microscopy samples
(1) Tissue and organ printing method:
Prepare the desired instrument and fixation, put it 4 ° C and pre-cooling in the refrigerator . Such as puncture (kidney tissue, liver tissue, spleen tissue, etc.), shearing (gastric mirror shearing, bronchoscopic mirror clipping, colonoscopy), surgical resection (tumor tissue, and experimental animal organ Wait, it should be quickly rolled with physiological saline after passing through physiological saline, or directly put into a precipitation 2.5 months, and put it 4 ° C in the refrigerator, generally required Completed within two minutes. When treating surgical resection, two sharp blades (such as razor or surgery) and wax plates should be prepared in advance, put the fixed droplets on the wax, and then cut the cutting organs into small The block is placed in a fixed liquid on the wax plate, and then uses a double blade tuning method (for details, please consult our staff) to cut the tissue into about 1 cubic millimeter size or cross-cut surface about 1 square millimeter. Long strip, then use toothpick or tweezers (gently clamped, prohibiting squeezing) to move the tissue into a small bottle equipped with a fixed liquid, and seal it with a medical elimination and tag. Such as the lung tissue, should be sufficiently oscillated in the fixed liquid to sink to the bottom of the bottle.
(2) Free cell access method:
tissue culture and free cells (such as blood cells, peatders, bacterial viruses, etc.), often suspended dispersion in the medium, first aggregate into groups, then fix, specifically The practice is as follows:
1, plasma agglutination: anti-coagulation whole blood centrifugation layer (2000 rpm), 20 minutes per minute, 10 u0026 mdash; with capillary straw along the tube wall, it will be absorbed (do not destroy The white blood cell layer) then sucks the fixing liquid with a pipette and slowly adds 2.5% glutaraldehyde fixation along the tube wall and then placed it in the 4 ° C refrigerator.
2, plasma (serum) mixed: such as bacterial, viruses, detonation cells, cultured cells, first formulate suspensions in the centrifuge (preferably glass), centrifugation (1000 ~ 3000 rot / Minute, 10 minutes, the same), after the supernatant is removed, 2.5% glutaraldehyde is fixed for about 10 minutes, centrifuge, and then discard the extra fixation, then mix uniform and mixed with a small amount of anticoagulant plasma or serum. To the supernatant (stay less), draw a 2.5% glutaraldehyde fixation with a pipette slowly dripped along the centrifuge tube wall, try to avoid the dispersion of the group, and stand in the 4 ° C refrigerator to save the spare.

Transmission electron microscopy test customers provide

tissue block, cell, etc.

The company provides

Basic experimental steps, drugs, sample processing, pictures and picture analysis

Experiment cycle: 2-3 Week