Shanghai Nelsi Dyeing Experiment

The use of Nelsonal Dyeing Experiment

The neuron is the structure and functional unit of the nervous system, and the NiT is one of the characteristic structures of the neurons. According to the tiger spots, there is a nerve cell body and a dendritic, which has basis. In the past, the neurotic nature of the neurotenthrism was used to use Sumu-Inghong staining method, although this method also observed the basophysical particles in the cytoplasm, but the structure was not very clear, the nuclei, nucleolore, Ni-body particles blur , The division is unclear, axon, and the dendritic is difficult to identify. In Nikitan Dye, NC, core, nuclear benevolence is also very clear, and it is easy to distinguish between axons and dendrites. It has received the effects of both identifiable organs and simultaneous observation of cytoplasmic structures.

Niki staining method and steps: slice deprnowled to water, immerse the slice 5 GPL sulfur aqueous solution, placed in a 37 ° C thermophoto. After removing the slice, the water was washed. After mixing the ethanol solution having a volume fraction of 0.70, 50 GPL Iranian Eragin solution is reset. The amount of the volume fraction is 0. 95, 0. 95, 1. 00, 1. 00, respectively, dehydrated. Transparent xylene, neutral gum. In the dyeing process, we experienced that after specimen into the aqueous solution, it should be solened, and the dyeing time of Iraqi is strictly controlled.

paraffin samples often maintain transport; freezing slice sample - 20 degrees preservation transport;

Test Cycle

15 days