Shanghai Morris Water Maze Experiment

Shanghai Morris Water Maze Test

Morris Water Maze Tes Ter is more classic in space learning memory capabilities, has been widely used in more than 30 years, experimental methods It is also constantly improving innovation, and the experiment object is the rat, and later expanded to mice or even human (virtual maze)

MrRIS research progress (Shanghai Morris water maze experiment)

Morris Water maze experiments are numerous, and more procedures in China are adaptive training, positioning navigation experiments and space exploration experiments. Adaptive training is the day before the experiment, no platform, allowing the large mouse freely swim 120s in the water maze, and put it in a warm and dry cage to avoid the stress response of the rats. The positioning navigation experiment is also a hidden platform experiment, mainly to investigate the animal's learning ability. When experiment, the large and medium mouse face pond into the water, record the escape latency of the large mice, the total movement of the movement, the target quadrant ratio and other indicators. If the ratio is not found in the specified time, the large mice will be guided to the platform to accommodate the location of the platform. Space Exploration Experiment Evaluate Animal Space Memory Ability. It is generally carried out after 24 hours of positioning navigation experiments, and the platform is removed, and the rats are free to swim, observe the number of larger rats, and the target quadrant ratio and other indicators investigated the memory capability of the mouse. In foreign countries, the reverse space experiment is more, and the reverse space experiment is to move the platform to 180 u0026 deg; the animal inlet is also adjusted to the original position 180 u0026 deg; the remainder is sailing. Experiment, spatial exploration experiment after 24 hours. The reverse space experiment has increased the difficulty of experiment, mainly to investigate the progress of MORRIS water maze experiments and precautions in Morris water maze

Precautions

1. Environment and experimenter's location in the laboratory can be used as a reference for rat search targets, so the position of the laboratory equipment and the experimenter should be relatively fixed.

2. The water maze laboratory should be quiet, soft and uniform.