Shanghai eight arm maze experiments.

Shanghai eight arm maze experiments. Calculation and data analysis

Evaluate animal memory, namely, Working u0026 Nbsp; Of Working u0026 Nbsp; Memory Errors) The reference memory error frequency (Frequency of Reference Memory Erro "is equal to the ratio of work memory errors or reference memory errors and total number of arms. The work memory and reference memories were evaluated by these two indicators. At the same time, the average exploration time is calculated, that is, the ratio of the test time and the total number of arms is calculated, which is an indicator of general motion activity. According to the damage of the destroyed brain structure or the drug weakened, the work memory error frequency and / or reference memory error frequency is significantly increased, and the memory enhancement drug or treatment can reduce this error frequency.
Shanghai eight arm maze experiments. Precautions

1. mouse radiosrination equipment and experimental procedures are similar to rats, but labyrinth specification should be 1/4 to 1/2 of rat maze, so as not to increase mice behavior.

2. This experiment can also be used to determine work memory. In the method, the * is different, and all food grains are placed in the radi arm, rather than only 4 arms are placed.

3. Chronic stress to the animal's maze operation can have an effect, and there is a gender difference. After chronic stress, the memory of male rats was reduced, and the frequency of memory error was increased; the spatial memory of male rats was reinforced, and the error frequency was reduced.

4. Even under the restriction feeding conditions, rat body weight should be increased by 5G per week to prevent animals from malnutrition. Animals that eliminate poor body state.

5. One item around the labyrinth can be used as a marker for space as space. Removing or moving these marks may make animal operation difficult and reduce the accuracy of the maze arms.

6. Different labyrinth radiobes depending on the experimental purpose can be different, including 8, 16, 24, 32, 40, and 48 arm maze. The less the maze arm requires that the animal remembers the arm is also smaller, the more simply operated the animal behavior operation. On the one hand, the number of additions increases the requirements for animal space memory, and on the other hand, more, and considering interference factors (eg, past maze learning on the current measurement memory). Therefore, it is usually used to radiate the maze using 8 arms, which can be reduced, too many arms, and shorten the time spent on training and testing.

7. Food used is usually small, with chocolate flavor (animal favorite taste) or sweetBreakfast (10 mg per piece); liquid food (such as chocolate milk or water). The latter is especially practical for testing some drugs that affect the swallowing of solid foods (such as east ###).

8. There are two major factors affecting animal maze operations: fear and animal exploration habits and animal exploratory habits and animals are known to be driven by food in the maze arm. Fear factors will prevent animal maze operations, so that animals always stay in a certain place in the maze without exploring. The lack of desire for food will also produce similar results, increase the touch of animals, and the side walls of the laminate arm are added to help reduce animal fear. If the driving effect of food is insufficient, it can reduce the amount of food, but simultaneously monitor weight and general physical condition. Usually, the weight should not be lower than 80% before the fastening; the weight is reduced by 15% for most rats.

9. Different with water maze, radiocar maze is suitable for repeated testing or long-term memory. It is generally believed that work memories represent short-term memory, and reference memory represents long-term memory.