Shanghai T labyrinth experiment rat and mouse have good spatial discrimination function, can be very fast Learn and accurately operate the maze. Therefore, the T-shaped maze and radiocarpine are widely used to test the spatial memory capability of animals. T-shaped maze is used to study the effects of different brain regions on spatial memory.
Shanghai T Maze Test Experimental Test

(1) T Maze appearance is u0026 ldquo; T u0026 RDQUO; shape, by A backbone and two select arms are composed of 60cm long, each arm is 30 cm long, 10 cm wide, 20cm high. There are 1 food cup in the end of the two selectors, used to put food (the reward food used in this experiment is sunflower seeds), and there is a pull-pull baffle at the proximal end of the select arm. The labyrinth is all black, so that the mice do not have a reference when getting food rewards, and can only choose one end of the correct food placed according to self-determination. In order to increase the driving force of the mouse to find food, 3 days of the test restriction (fasting 6 h), the weight is reduced to 80% -90% of the normal acquisition of food. And the first training before the test was tested, so that mice familiar with the environment in the maze, so they can find food.

(2) Experiments were carried out in a quiet room, and environmental conditions were stable. At the time of the test, * stepped in the maze, only allowed the animal to reach another arm of the T maze, get food in this animal; in the second step, open the two arms of the T labyrinth, but only have been blocked There is food in the arm, and mice only choose to enter the opposite to * step to get food, remember to be correct, such as mice re-entry * The arm has been previously removed. Such two steps are a group, two step experimental intervals 15 s. Each mouse performs 10 or 15 groups of experiments per day, record its correct selection, total test 3 d.