Shanghai 旷场 experiment
[Experimental purpose]
The analysis system is observing the study of experimental animal neuropsychia, enters the open environment, such as animals to new The environmental fear is mainly in the surrounding area, and there is less in central region, but the exploration characteristics of animals have caused them to produce motives in central regional activities, and it can observe the anxiety psychology generated by this.
Shanghai 旷场 experimental experimental step
1. Experiments in a quiet environment;
2. Behavioral indicator assay animal Spontaneous activities. Grab the rat or mouse tail root, gently put it into the wild experiment box, simultaneous imaging and timing;
3. Stop the camera after observation time, observation time According to the experiment, generally 3 to 5 min;
4. Record 3-5 min within 3-5 min, the number of dialogs, the number of dial, the number of erection, the number of rejection, and the number of rejection of the rejection. Experiments are single blind;
5. Cleaning the inner wall of the square box and the bottom surface, so as not to wait for the amount of information remaining in the animal (such as the animal's large, urination, odor) affects the next test results. Replace the animal and continued experiment;
6. Statistical treatment Experimental results are expressed in S, inter-group difference uses variance analysis, with STUDENT T test.
1. Animals have their activity cycles within 24 h, so each experiment should be selected within the same time period.
2. The experiment should be carried out in a behavior laboratory of sound insulation, light intensity and temperature, humidity and maintain *.
3. Cleaning the experiment between the experiments to avoid the next experimental results.[123 ]