Shanghai Kong Kong Experiment
Shanghai Kong Due experiment
( ) One
Basic principle Decorator experiment is Boissiex
andSIMON1962 [ 123] Established, it was widely used in pharmacological research. This experiment is to use novel (Curiosity) and fear (FEAR) to control the behavior of animals in a new environment. The results of these two factors were reflected by escaping (ESCAPES) . Animal repeated drill (Head Dipping) reflects its new sense and the desire to escape. It is generally believed that drugs increasing the number of drills without affecting animal motion activity, exhibiting an anti-anxiety effect, reducing the number of drills and time, as a result. Shanghai hole experiment operation step Experiment of rat and mice The device is different. The rat device is a 66cm x 56cm x 47cm
of 4 a diameter is 3. 8cm
, deep of the large round hole, two of which are close to the near wall 14cm , Another two holes 17CM , the hole plate level is raised 12EM; mouse wooden box is 44CM x 40cm X 27cm, 4 3CM , thick 1. 8cm The distance from the center of each well is L0CM . 80 After the era, there is an infrared light around the hole and each well.The electric tube is associated with the microcomputer, which can automatically record multiple indicators of the number of drills of animals and the number of drills, motion activity and standing number, both improve work efficiency and increase the reliability of the experiment. Experimental male adult rat or mice. Place the animals in the center of the orifice plate and facing the observer. Animals disappear in the cave for a drill bit (A Head DIP) , record 5- 10min The number of drills and drills duration. . ( 1 ) There is no uniform standard for the shape and size of the orifice box, but is often used, and the side length is generally in rats 60CM, mice 40C m . (2)
The number of holes is mostly 16 holes, and now
Dellar.(3) Animals are often more than afternoon. Therefore, it should be tried to do experiments in a fixed time per day. (4) The experiment is for anxiety ( , such as BDZ
Receptor inverted agonist) causes the specific reduction result of the inquiry drill bit *, and some drugs that lack an anti-anxiety effect [ ( BDZ receptor antagonist fluorimethi ) Conversion to the inquiry drill bit Increase. Therefore, when screening unknown drugs should be evaluated in conjunction with other anxiety models.