Shanghai spontaneous activities experiment

u0026 nbsp ; u0026; Motion distance, motion speed, etc. It is in an enclosed environment, no light, and is measured in infrared. This experiment is called Locomotor Activity.

Principle: Spontaneous activities are physiological characteristics of normal animals. How many spontaneous activities can often reflect the statute or inhibitory effect of the center. Central inhibitory drugs such as sedative hypnotics can significantly reduce spontaneous activities in mice; and the central and warm pharmaceuticals can increase the spontaneous activities of mice. The degree of spontaneous activity increase in mice is proportional to the intensity of the central excitement or inhibitor.

Experimental materials: mouse, reagent, kit, sodium benzoate *** solution

Instrument, consumable, clock cover, syringe, balance Experimental step

First, method:

Take a small mouse 2 Only, weigh the weight, put it in two bells. After the mouse was quiet, the abdominal injection was carried out, one 1% benzoate *** solution 150 mg / kg, and another 0.5% stamp 20 mg / kg. After 10 minutes of administration, the activities of two mice were observed, what is more different. Precautions

⒈ Small white rats should try to select the active, same sex.

⒉ Fasting for 24 h before the experiment.

⒊ Experiment is good at room temperature at room temperature at room temperature, and the mouse activity is often affected by the room temperature.

⒋ Experiment is good in the morning, thinking that the activity of mice is usually reduced at noon and afternoon.

⒌ observation content such as walking, standing, sniffing, impression, licking, biting, balance disorder, tail, etc.