Shanghai Mingxia Experiment

(black and white box) experiment (

Light-Dark u0026 u200b u200bNbsp; Transspitation TEST [123 1. u0026 Nbsp; Shanghai Mingbox Experiment Basic Principle

In the light In the box, mice or rats like in the dark box activity, but the animal's exploration habits have prompted them to try to explore the box. However, the bright stimulation of the bright box and inhibits the exploration of the animal in the bright box. Anti-anxiety drug can relieve this inhibitory effect. II. u0026 Nbsp; Operation step

bright and dark box (

45cm * 27cm * 27cm ) In the dark box, the top is covered; the bright box accounts for two-thirds, highlighting, the partition between the two boxes has a [123

of the door hole for animals. The bright dark box is placed in On the actuator, the motion activity of the animal can be recorded at the same time. When the animal was administered, it was placed in the center of the bright box, and 10 min The number of passes and the retention time distributed in the bright box and the darkbox. Shimada In the same year, the device of the bright and dark box was bold Improved, in one 40CM * 40CM * 12CM 10cm * 10cm * 12cm [

of the dark box, thus constitutes the other two-diagonal u0026 ldquo; L u0026 rdquo; shaped runway (bright box), each There is an The hole is connected to the runway on both sides. Above the device is equipped with a television scan and an image pickup system, thereby observing the number of wearing bins in mice, in the macro hook time and motion activity. Three. u0026 Nbsp; Items and evaluation 1. Anti-anxiety drug (***, pentobital and sleeping, etc.) can increase the wearing box of animalsThe number of times and the retention time in the mutant box, the non-anxiety agent has no effect. 2. The relative use intensity and clinical trial result of the anti-anxious agent on this model *. 3. SHIMADA Improved bright and dark box experiments are sensitive, whether it is a classic antimusion or anhydration, or a new anti-anxiety such as butchidoneThe agent is positive in this model.