Shanghai cell proliferation experiment

Shanghai Cell Proliferation Experiment ] cell proliferation is an important life characteristics of organisms, and cells proliferate in a division. Single cell organisms produce new individuals in a cell division. Multi-cell organisms produce new cells in a cell division, used to supplement aging or dead cells in vitro.
Shanghai Cell Proliferation Experiment Test Method

Apply MTT colorimetric, detect cell survival and growth. Its detection principle is the succinate dehydrogenase in the living cells in the living cells to form a blue crystalline Formazan to form blue crystallization formazan, and are deposited in the cell, and the dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve Formazan in cells, and use an enzyme-linked immunoassay to measure its light absorption value at a wavelength, indirectly reflect the number of live cells. In the range of cells, the amount of MTT crystal formation is proportional to the number of cells. In addition, the CCK8 method is also common method .

The OCT-4 overtensed cell group was quantified by the CCK-8 method, and the empty cell group was quantified to the cell proliferation of the negative control group to study the effects of OCT-4 gene on the proliferation of 293T cells. The experimental results show that the expression of OCT-4 gene promotes proliferation capabilities of 293T cells. Provide experimental data to further study the function of the OCT-4 gene. these two methods have been widely used in some activity detection of biological active factors, large-scale anti-tumor drug screening, cytotoxic test, And tumor radiological sensitivity assays, etc. NBSP;